Current and former members of the group highlighted in bold font
Pre-prints or in revision:
- L. Potenza, L. Kozon, L. Drewniak, T.S. Kaminski. Passive droplet microfluidic platform for high-throughput screening of microbial proteolytic activity, submitted
- L. Kozon, L. Derzsi, P. Garstecki, T. Kaminski. SpotVac Chip: a vacuum-driven microfluidic device for the multiplex screening of antibiotic susceptibility, submitted
- Z. Olszewska*, A. Pyzik*, M. Małkowski* (*co-first authors), T.S. Kaminski. Droplet microfluidic systems for multistep single-cell sequencing assays. accepted
- M. Gantz, S. V. Mathis, F. E. H. Nintzel, P.J. Zurek, T. Knaus, E. Patel, D. Boros, F.-M. Weberling, M. R. A. Kenneth, O. J. Klein, E.J. Medcalf, J. Moss, M. Herger, T. S. Kaminski, F. G. Mutti, P. Lio, F. Hollfelder. Microdroplet screening rapidly profiles a biocatalyst to enable its AI-assisted engineering. bioRxiv, doi.org/10.1101/2024.04.08.588565, in revision.
- K. Fischer, A. Lulla, T. So, P. Pereyra-Gerber, M.I.J. Raybould, T.N. Kohler, T. S. Kaminski, J. Carlos Yam-Puc, R. Hughes, F. Leiss-Maier, P. Brear, N.J. Matheson, C. Deane, M. Hyvonen, J. Thaventhiran, F. Hollfelder. Microfluidics-enabled fluorescence-activated cell sorting of single pathogen-specific antibody secreting cells for the rapid discovery of monoclonal antibodies. bioRxiv, doi.org/10.1101/2023.01.10.523494, in revision in Nature Biotechnology.
Published:
- R. Scheele, Y. Weber, F. Nintzel, M.Herger, T. S Kaminski, F. Hollfelder. Ultrahigh throughput evolution of tryptophan synthase in droplets via an aptamer-biosensor. ACS Catalysis 2024, 14, 8, 6259–6271.
- K. Staskiewicz, M. Dabrowska, L. Kozon, Z. Olszewska, L. Drewniak, T. S. Kaminski. Droplet Microfluidic System for High Throughput and Passive Selection of Bacteria Producing Biosurfactants. Lab Chip, 2024, 24, 1947-1956.
- M. A. Iwan, M. Günthel, T.S. Kaminski, W. Franus, Ł. Drewniak. Biotransformation of coal fly ash with high carbon content; behavior and release patterns of coal fly ash constituents under the influence of allochtonic Pseudomonas stutzer MT1. Journal of Cleaner Production, 2023, 420, 138358.
- J. De Jonghe*, T.S. Kaminski* (*co-first authors), D. B. Morse, M. Tabaka, A. L. Ellermann, T. N. Kohler, G. Amadei, C. Handford, G.M. Findlay, M. Zernicka-Goetz, F. Hollfelder. spinDrop: a droplet microfluidic platform to maximise single-cell sequencing information content. Nature Communications, 2023, 14, 4788.
- S. Ladeveze, P.J. Zurek, T. S. Kaminski, S. Emond, F, Hollfelder. Versatile Product Detection Via Coupled Assays for Ultra-High-Throughput Screening of Carbohydrate-Active-Enzymes in Microfluidic Droplets. ACS Catalysis, 2023, 13, 15, 10232–10243.
- E.J. Medcalf, M. Gantz, T.S. Kaminski*, F. Hollfelder* (*co-corresponding author). Ultrahigh-throughput Absorbance Activated Droplet Sorting (UHT-AADS) for enzyme screening at kilohertz frequencies. Analytical Chemistry, 2023, 95, 10, 4597–4604.
- D.S. Fernández, O.J. Klein, T.S. Kaminski, P.Y. Colin, F. Hollfelder. Ultrahigh-throughput directed evolution of a metal-free α/β-hydrolase with a Cys-His-Asp triad into an efficient phosphotriesterase. Journal of the American Chemical Society, 2023, 145, 2, 1083–1096.
- B. D. G. Eeenink, T.S. Kaminski, E. Bornberg-Bauer, J. Jose, F. Hollfelder, B. van Loo. Vector Redesign and In-Droplet Cell-Growth Improves Enrichment and Recovery in live Escherichia coli. Microbial Biotechnology, 2022, 15, 2845-2853.
- F. Salmen, J. De Jonghe, T. S. Kaminski, A. Alemany, G. Parada, J. Verity-Legg, A. Yanagida, T. N Kohler, N. Battich, F. van den Brekel, A. L. Ellermann, A. Martinez-Arias, J. Nichols, M. Hemberg, F. Hollfelder, A. van Oudenaarden. High-throughput total RNA sequencing in single cells using VASA-seq. Nature Biotechnology, 2022, 40, 1780–1793.
- M. Klaus, P.J. Zurek, T.S. Kaminski, A. Pushpanath, K. Neufeld, F. Hollfelder. Ultrahigh-Throughput Detection of Enzymatic Alcohol Dehydrogenase Activity in Microfluidic Droplets with a Direct Fluorogenic Assay. ChemBioChem 2021, 22, 3292 –3299.
- Y.T. Kao, T.S. Kaminski, W. Postek, J. Guzowski, K. Makuch, A. Ruszczak, A. von Stetten, R. Zengerle, P. Garstecki. Gravity-driven microfluidic assay for digital enumeration of bacteria and for antibiotic susceptibility testing. Lab on a Chip, 2020, 20, 54-63.
- M. A. Czekalska, T.S. Kaminski, K. Makuch, P. Garstecki. Passive and parallel microfluidic formation of droplet interface bilayers (DIBs) for measurement of leakage of small molecules through artificial phospholipid membranes. Sensors and Actuators B: Chemical, 2019, 286, 258-265.
- A. Opalski, T. S. Kaminski, P. Garstecki. Droplet microfluidics as a tool for generation of granular matters and functional emulsions. KONA Powder and Particle Journal, 2019, 2019004.
- W. P. Postek, P. Gargulinski, O. Scheler, T. S. Kaminski, P. Garstecki. Microfluidic screening of antibiotic susceptibility at a single-cell level shows inoculum effect of cefotaxime in E. coli. Lab on a Chip, 2018, 18, 3668-3677.
- T. S. Kaminski*, P. Garstecki* (*co-corresponding author). Controlled droplet microfluidic systems for multistep chemical and biological assays. Chemical Society Reviews, 2017, 46, 6210-6226.
- Cover article
- W. Postek, T. S. Kaminski, P. Garstecki. A precise and accurate microfluidic droplet dilutor. Analyst, 2017, 142, 2901-2911.
- O. Scheler, N. Pacocha, P.R. Debski, A. Ruszczak, T. S. Kaminski, P. Garstecki. Optimized droplet digital CFU assay (ddCFU) provides precise quantification of bacteria over dynamic range of 6 logs and beyond. Lab on a Chip, 2017, 17, 1980-1987.
- M. A. Czekalska, T.S. Kaminski, M. Horka, S. Jakieła, P. Garstecki. An automated microfluidic system for generation of Droplet Interface Bilayers networks. Micromachines, 2017, 8, 93.
- W. Postek, T. S. Kaminski, P. Garstecki. A passive microfluidic system based on step emulsification allows the generation of libraries of nanoliter-sized droplets from microliter droplets of varying and known concentrations of a sample. Lab on a Chip, 2017, 17, 1323-1331.
- T. Sewastianik, M. Szydlowski, E. Jablonska, E. Bialopiotrowicz, P. Kiliszek, P. Gorniak, A. Polak, M. Prochorec-Sobieszek, A. Szumera-Cieckiewicz, T. S. Kaminski, S. Markowicz, E. Nowak, M. A. Grygorowicz, K. Warzocha, P. Juszczynski. FOXO1 is a TXN- and p300-dependent sensor and effector of oxidative stress in diffuse large B-cell lymphomas characterized by increased oxidative metabolism. Oncogene, 2016 35, 5989–6000.
- T. S. Kaminski*, O. Scheler*, (*equal contribution), P. Garstecki. Droplet microfluidics for microbiology: techniques, applications and challenges. Lab on a Chip, 2016, 16, 2168-2187.
- O. Scheler, T. S. Kaminski, A. Ruszczak, P. Garstecki. Dodecylresorufin (C12R) outperforms resorufin in micro-droplet bacterial assays. ACS Appl. Mater. Interfaces, 2016, 8, 11318–11325.
- L. Derzsi*, T. S. Kaminski* (*equal contribution), P. Garstecki. Antibiograms in five pipetting steps: precise dilution assays in sub-microliter volumes with a conventional pipette. Lab on a Chip, 2016, 16, 893-901.
- P. Debski, K. Gewartowski, M. Sulima, T. S. Kaminski, P. Garstecki. Rational design of digital assays. Analytical Chemistry, 2015, 87 (16), 8203–8209.
- M. Czekalska*, T. S. Kaminski* (*equal contribution), S. Jakiela, K. T. Sapra, H, Bayley, P. Garstecki. A droplet microfluidic system for sequential generation of lipid bilayers and transmembrane electrical recordings. Lab On a Chip, 2015, 15, 541-548.
- S. Hou, L. Tabaka M., Sun, P. Trochimczyk, T. S. Kaminski, T. Kalwarczyk, Zhang X. R. Holyst. A Flexible Fluorescence Correlation Spectroscopy Based Method for Quantification of the DNA Double Labelling Efficiency with Precision Control. Laser Physics Letters, 2014, 11, 085602.
- S. Hou, L. Sun, S. A. Wieczorek, T. Kalwarczyk, T. S. Kaminski, R. Holyst. Fluorescence correlation spectroscopy analysis for accurate determination of proportion of doubly labeled DNA in fluorescent DNA pool for quantitative biochemical assays. Biosensors and Bioelectronics, 2014, 51, 8-15.
- S. Jakiela*, T. S. Kaminski* (*equal contribution), O. Cybulski, D.B. Weibel, P. Garstecki. Bacterial growth and adaptation in microdroplet chemostats. Angewandte Chemie Int. Ed., 2013, 52, 8908-8911.
- T. S. Kaminski, S. Jakiela, M. Czekalska, W. Postek P. Garstecki. Automated and rapid generation of libraries of nL droplets. Lab on a Chip, 2012, 12, 3995-4002.
- S. Hou, S. A. Wieczorek, T. S. Kaminski,N. Ziebacz, M. Tabaka, N. A. Sorto, M. H. Foss, J. T. Shaw, M. Thanbichler, D. B. Weibel, R. Holyst, P. Garstecki.Characterization of Caulobacter crescentus FtsZ.
- K. Churski, T. S. Kaminski, S. Jakiela, W. Kamysz, W. Baranska-Rybak, D. B. Weibel, P. Garstecki.Rapid screening of antibiotic toxicity in an automated microdroplet system. Lab On a Chip, 2012, 12, 1629 – 1637.
- S. Hou, N. Ziebacz, S.A. Wieczorek, E. Kalwarczyk, V. Sashuk, T. Kalwarczyk, T. S. Kaminski, R. Holyst. Formation and structure of PEI/DNA complexes: quantitative analysis. Soft Matter, 2011, 7, 6967-6972.
- S. Hou, N. Ziebacz, T. Kalwarczyk, T. S. Kaminski, S.A. Wieczorek, R. Holyst. Influence of Nano-viscosity and Depletion Interactions on Cleavage of DNA by Enzymes in Glycerol and Poly(ethylene) Glycol Solutions: Qualitative Analysis. Soft Matter, 2011, 7, 3092-3099.
Book chapters:
- S. Neun*, P. Zurek*, T. S. Kaminski* (*equal contribution), F. Hollfelder. Ultrahigh throughput screening for enzyme function in droplets in: Enzyme Engineering and Evolution: General Methods, 2019, 643, 317
- S. Neun*, T. S. Kaminski* (*equal contribution), F. Hollfelder. Single-cell activity screening in microfluidic droplets in: Methods in Enzymology, 2019, 628, 95-112
- T. S. Kaminski, K. Churski, P. Garstecki. Automated droplet microfluidic chips for biochemical assays in: Microdroplet technology and microfluidics: principles, components, and emerging applications in biology and chemistry. Editors: P. Day, A. Manz, Y. Zhang. Springer 28 July 2012 ISBN: 978-1461432647.