Current and former members of the group highlighted in bold font

Pre-prints or in revision:

  • L. Potenza, L. Kozon, L. Drewniak, T.S. Kaminski. Passive droplet microfluidic platform for high-throughput screening of microbial proteolytic activity, submitted
  • L. Kozon, L. Derzsi, P. Garstecki, T. Kaminski. SpotVac Chip: a vacuum-driven microfluidic device for the multiplex screening of antibiotic susceptibility, submitted
  • Z. Olszewska*, A. Pyzik*, M. Małkowski* (*co-first authors), T.S. Kaminski. Droplet microfluidic systems for multistep single-cell sequencing assays. accepted
  • M. Gantz, S. V. Mathis, F. E. H. Nintzel, P.J. Zurek, T. Knaus, E. Patel, D. Boros, F.-M. Weberling, M. R. A. Kenneth, O. J. Klein, E.J. Medcalf, J. Moss, M. Herger, T. S. Kaminski, F. G. Mutti, P. Lio,  F. Hollfelder. Microdroplet screening rapidly profiles a biocatalyst to enable its AI-assisted engineering. bioRxiv,, in revision.
  • K. Fischer, A. Lulla, T. So, P. Pereyra-Gerber, M.I.J. Raybould, T.N. Kohler, T. S. Kaminski, J. Carlos Yam-Puc, R. Hughes, F. Leiss-Maier, P. Brear, N.J. Matheson, C. Deane, M. Hyvonen, J. Thaventhiran, F. Hollfelder. Microfluidics-enabled fluorescence-activated cell sorting of single pathogen-specific antibody secreting cells for the rapid discovery of monoclonal antibodies. bioRxiv,, in revision in Nature Biotechnology.


  1. R. Scheele, Y. Weber, F. Nintzel, M.Herger, T. S Kaminski, F. Hollfelder. Ultrahigh throughput evolution of tryptophan synthase in droplets via an aptamer-biosensor. ACS Catalysis 2024, 14, 8, 6259–6271.
  2. K. Staskiewicz, M. Dabrowska, L. Kozon, Z. Olszewska, L. Drewniak, T. S. Kaminski. Droplet Microfluidic System for High Throughput and Passive Selection of Bacteria Producing Biosurfactants. Lab Chip, 2024, 24, 1947-1956.
  3. M. A. Iwan, M. Günthel, T.S. Kaminski, W. Franus, Ł. Drewniak. Biotransformation of coal fly ash with high carbon content; behavior and release patterns of coal fly ash constituents under the influence of allochtonic Pseudomonas stutzer MT1. Journal of Cleaner Production, 2023, 420, 138358.
  4. J. De Jonghe*, T.S. Kaminski* (*co-first authors), D. B. Morse, M. Tabaka, A. L. Ellermann, T. N. Kohler, G. Amadei, C. Handford, G.M. Findlay, M. Zernicka-Goetz, F. Hollfelder. spinDrop: a droplet microfluidic platform to maximise single-cell sequencing information content. Nature Communications, 2023, 14, 4788.
  5. S. Ladeveze, P.J. Zurek, T. S. Kaminski, S. Emond, F, Hollfelder. Versatile Product Detection Via Coupled Assays for Ultra-High-Throughput Screening of Carbohydrate-Active-Enzymes in Microfluidic Droplets. ACS Catalysis, 2023, 13, 15, 10232–10243.
  6. E.J. Medcalf, M. Gantz, T.S. Kaminski*, F. Hollfelder* (*co-corresponding author). Ultrahigh-throughput Absorbance Activated Droplet Sorting (UHT-AADS) for enzyme screening at kilohertz frequencies. Analytical Chemistry, 2023, 95, 10, 4597–4604.
  7. D.S. Fernández, O.J. Klein, T.S. Kaminski, P.Y. Colin, F. Hollfelder. Ultrahigh-throughput directed evolution of a metal-free α/β-hydrolase with a Cys-His-Asp triad into an efficient phosphotriesterase. Journal of the American Chemical Society, 2023, 145, 2, 1083–1096.
  8. B. D. G. Eeenink, T.S. Kaminski, E. Bornberg-Bauer, J. Jose, F. Hollfelder, B. van Loo. Vector Redesign and In-Droplet Cell-Growth Improves Enrichment and Recovery in live Escherichia coli. Microbial Biotechnology, 2022, 15, 2845-2853.
  9. F. Salmen, J. De Jonghe, T. S. Kaminski, A. Alemany, G. Parada, J. Verity-Legg, A. Yanagida, T. N Kohler, N. Battich, F. van den Brekel, A. L. Ellermann, A. Martinez-Arias, J. Nichols, M. Hemberg, F. Hollfelder, A. van Oudenaarden. High-throughput total RNA sequencing in single cells using VASA-seq. Nature Biotechnology, 2022, 40, 1780–1793.
  10. M. Klaus, P.J. Zurek, T.S. Kaminski, A. Pushpanath, K. Neufeld, F. Hollfelder. Ultrahigh-Throughput Detection of Enzymatic Alcohol Dehydrogenase Activity in Microfluidic Droplets with a Direct Fluorogenic Assay. ChemBioChem 2021, 22, 3292 –3299.
  11. Y.T. Kao, T.S. Kaminski, W. Postek, J. Guzowski, K. Makuch, A. Ruszczak, A. von Stetten, R. Zengerle, P. Garstecki. Gravity-driven microfluidic assay for digital enumeration of bacteria and for antibiotic susceptibility testing. Lab on a Chip, 2020, 20, 54-63.
  12. M. A. Czekalska, T.S. Kaminski, K. Makuch, P. Garstecki. Passive and parallel microfluidic formation of droplet interface bilayers (DIBs) for measurement of leakage of small molecules through artificial phospholipid membranes. Sensors and Actuators B: Chemical, 2019, 286, 258-265.
  13. A. Opalski, T. S. Kaminski, P. Garstecki. Droplet microfluidics as a tool for generation of granular matters and functional emulsions. KONA Powder and Particle Journal, 2019, 2019004.
  14. W. P. Postek, P. Gargulinski, O. Scheler, T. S. Kaminski, P. Garstecki. Microfluidic screening of antibiotic susceptibility at a single-cell level shows inoculum effect of cefotaxime in E. coli. Lab on a Chip, 2018, 18, 3668-3677.
  15. T. S. Kaminski*, P. Garstecki* (*co-corresponding author). Controlled droplet microfluidic systems for multistep chemical and biological assays. Chemical Society Reviews, 2017, 46, 6210-6226.
    • Cover article
  16. W. Postek, T. S. Kaminski, P. Garstecki. A precise and accurate microfluidic droplet dilutor. Analyst, 2017, 142, 2901-2911.
  17. O. Scheler, N. Pacocha, P.R. Debski, A. Ruszczak, T. S. Kaminski, P. Garstecki. Optimized droplet digital CFU assay (ddCFU) provides precise quantification of bacteria over dynamic range of 6 logs and beyond. Lab on a Chip, 2017, 17, 1980-1987.
  18. M. A. Czekalska, T.S. Kaminski, M. Horka, S. Jakieła, P. Garstecki. An automated microfluidic system for generation of Droplet Interface Bilayers networks. Micromachines, 2017, 8, 93.
  19. W. Postek, T. S. Kaminski, P. Garstecki.  A passive microfluidic system based on step emulsification allows the generation of libraries of nanoliter-sized droplets from microliter droplets of varying and known concentrations of a sample. Lab on a Chip, 2017, 17, 1323-1331.
  20. T. Sewastianik, M. Szydlowski, E. Jablonska, E. Bialopiotrowicz, P. Kiliszek, P. Gorniak, A. Polak, M. Prochorec-Sobieszek, A. Szumera-Cieckiewicz, T. S. Kaminski, S. Markowicz, E. Nowak, M. A. Grygorowicz, K. Warzocha, P. Juszczynski. FOXO1 is a TXN- and p300-dependent sensor and effector of oxidative stress in diffuse large B-cell lymphomas characterized by increased oxidative metabolism. Oncogene, 2016 35, 5989–6000.
  21. T. S. Kaminski*, O. Scheler*, (*equal contribution),  P. Garstecki. Droplet microfluidics for microbiology: techniques, applications and challenges. Lab on a Chip, 2016, 16, 2168-2187.
  22. O. Scheler, T. S. Kaminski, A. Ruszczak, P. Garstecki. Dodecylresorufin (C12R) outperforms resorufin in micro-droplet bacterial assays.  ACS Appl. Mater. Interfaces, 2016, 8, 11318–11325.
  23. L. Derzsi*, T. S. Kaminski* (*equal contribution), P. Garstecki. Antibiograms in five pipetting steps: precise dilution assays in sub-microliter volumes with a conventional pipette. Lab on a Chip, 2016, 16, 893-901.
  24. P. Debski, K. Gewartowski, M. Sulima, T. S. Kaminski, P. Garstecki. Rational design of digital assays. Analytical Chemistry, 2015, 87 (16), 8203–8209.
  25. M. Czekalska*, T. S. Kaminski* (*equal contribution), S. Jakiela, K. T. Sapra, H, Bayley, P. Garstecki. A droplet microfluidic system for sequential generation of lipid bilayers and transmembrane electrical recordings. Lab On a Chip, 2015, 15, 541-548.
  26. S. Hou, L. Tabaka M., Sun, P. Trochimczyk, T. S. Kaminski, T. Kalwarczyk, Zhang X. R. Holyst. A Flexible Fluorescence Correlation Spectroscopy Based Method for Quantification of the DNA Double Labelling Efficiency with Precision Control. Laser Physics Letters, 2014, 11, 085602.
  27. S. Hou, L. Sun, S. A. Wieczorek, T. Kalwarczyk, T. S. Kaminski, R. Holyst. Fluorescence correlation spectroscopy analysis for accurate determination of proportion of doubly labeled DNA in fluorescent DNA pool for quantitative biochemical assays. Biosensors and Bioelectronics, 2014, 51, 8-15.
  28. S. Jakiela*, T. S. Kaminski* (*equal contribution), O. Cybulski, D.B. Weibel, P. Garstecki. Bacterial growth and adaptation in microdroplet chemostats. Angewandte Chemie Int. Ed., 2013, 52, 8908-8911.
  29. T. S. Kaminski, S. Jakiela, M. Czekalska, W. Postek P. Garstecki. Automated and rapid generation of libraries of nL droplets. Lab on a Chip, 2012, 12, 3995-4002.
  30. S. Hou, S. A. Wieczorek, T. S. Kaminski,N. Ziebacz, M. Tabaka, N. A. Sorto, M. H. Foss, J. T. Shaw, M. Thanbichler, D. B. Weibel, R. Holyst, P. Garstecki.Characterization of Caulobacter crescentus FtsZ.
  31. K. Churski, T. S. Kaminski, S. Jakiela, W. Kamysz, W. Baranska-Rybak, D. B. Weibel, P. Garstecki.Rapid screening of antibiotic toxicity in an automated microdroplet system. Lab On a Chip, 2012, 12, 1629 – 1637.
  32. S. Hou, N. Ziebacz, S.A. Wieczorek, E. Kalwarczyk, V. Sashuk, T. Kalwarczyk, T. S. Kaminski, R. Holyst. Formation and structure of PEI/DNA complexes: quantitative analysis. Soft Matter, 2011, 7, 6967-6972.
  33. S. Hou, N. Ziebacz, T. Kalwarczyk, T. S. Kaminski, S.A. Wieczorek, R. Holyst. Influence of Nano-viscosity and Depletion Interactions on Cleavage of DNA by Enzymes in Glycerol and Poly(ethylene) Glycol Solutions: Qualitative Analysis. Soft Matter, 2011, 7, 3092-3099.

Book chapters:

  1. S. Neun*, P. Zurek*, T. S. Kaminski* (*equal contribution), F. Hollfelder. Ultrahigh throughput screening for enzyme function in droplets in: Enzyme Engineering and Evolution: General Methods, 2019, 643, 317
  2. S. Neun*, T. S. Kaminski* (*equal contribution), F. Hollfelder. Single-cell activity screening in microfluidic droplets in: Methods in Enzymology, 2019, 628, 95-112
  3. T. S. Kaminski, K. Churski, P. Garstecki. Automated droplet microfluidic chips for biochemical assays in: Microdroplet technology and microfluidics: principles, components, and emerging applications in biology and chemistry. Editors: P. Day, A. Manz, Y. Zhang. Springer 28 July 2012 ISBN: 978-1461432647.